I think this is a misunderstanding. I don't read

I think this is a misunderstanding. I don't read The Lancet. Are any of these papers satisfactory?


Isolation of infectious SARS-CoV-2 from urine of a COVID-19 patient

Jing Sun, Airu Zhu, Heying Li, Kui Zheng, Zhen Zhuang, Zhao Chen, Yongxia Shi, Zhaoyong Zhang, Si-Bei Chen, Xuesong Liu, Jun Dai, Xiaobo Li, Shuxiang Huang, Xiaofang Huang, Ling Luo, Liyan Wen, Jianfen Zhuo, Yuming Li, Yanqun Wang, Lu Zhang, Yanjun Zhang, Fang Li, Liqiang Feng, Xinwen Chen, Nanshan Zhong, Zifeng Yang, Jicheng Huang, Jincun Zhao, Yi-Min Li

Emerging microbes & infections 9 (1), 991-993, 2020

SARS-CoV-2 caused a major outbreak of severe pneumonia (COVID-19) in humans. Viral RNA was detected in multiple organs in COVID-19 patients. However, infectious SARS-CoV-2 was only isolated from respiratory specimens. Here, infectious SARS-CoV-2 was successfully isolated from urine of a COVID-19 patient. The virus isolated could infect new susceptible cells and was recognized by its’ own patient sera. Appropriate precautions should be taken to avoid transmission from urine.

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Virus isolation from the first patient with SARS-CoV-2 in Korea

Wan Beom Park, Nak-Jung Kwon, Su-Jin Choi, Chang Kyung Kang, Pyoeng Gyun Choe, Jin Yong Kim, Jiyoung Yun, Gir-Won Lee, Moon-Woo Seong, Nam Joong Kim, Jeong-Sun Seo, Myoung-don Oh

Journal of Korean medical science 35 (7), 2020

Novel coronavirus (SARS-CoV-2) is found to cause a large outbreak started from Wuhan since December 2019 in China and SARS-CoV-2 infections have been reported with epidemiological linkage to China in 25 countries until now. We isolated SARS-CoV-2 from the oropharyngeal sample obtained from the patient with the first laboratory-confirmed SARS-CoV-2 infection in Korea. Cytopathic effects of SARS-CoV-2 in the Vero cell cultures were confluent 3 days after the first blind passage of the sample. Coronavirus was confirmed with spherical particle having a fringe reminiscent of crown on transmission electron microscopy. Phylogenetic analyses of whole genome sequences showed that it clustered with other SARS-CoV-2 reported from Wuhan.

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Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells

Shutoku Matsuyama, Naganori Nao, Kazuya Shirato, Miyuki Kawase, Shinji Saito, Ikuyo Takayama, Noriyo Nagata, Tsuyoshi Sekizuka, Hiroshi Katoh, Fumihiro Kato, Masafumi Sakata, Maino Tahara, Satoshi Kutsuna, Norio Ohmagari, Makoto Kuroda, Tadaki Suzuki, Tsutomu Kageyama, Makoto Takeda

Proceedings of the National Academy of Sciences 117 (13), 7001-7003, 2020

A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating SARS-CoV-2. Our results reveal that, in common with SARS- and Middle East respiratory syndrome-CoV, SARS-CoV-2 infection is enhanced by TMPRSS2.

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SARS-CoV-2 virus isolated from the mastoid and middle ear: implications for COVID-19 precautions during ear surgery

KM Frazier, JE Hooper, HH Mostafa… - … –Head & Neck Surgery, 2020 - jamanetwork.com

Methods| The Johns Hopkins Hospital research autopsy program4 includes institutional review board-approved autopsy of COVID-19–positive (nasal swab, Cepheid GeneXpert SARS-CoV-2 assay) decedents, subject to safety limitations including avoidance of powered …

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Isolation of infectious SARS-CoV-2 from urine of a COVID-19 patient

J Sun, A Zhu, H Li, K Zheng, Z Zhuang… - Emerging microbes & …, 2020 - Taylor & Francis

… RNA has been detected in the sink and toilet of COVID-19 patients, the … and Testis May Cause Kidney and Testis Damage After 2019-nCoV Infection … and Personal Protective Equipment Contamination by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) From a …

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Virus isolation from the first patient with SARS-CoV-2 in Korea

WB Park, NJ Kwon, SJ Choi, CK Kang… - Journal of Korean …, 2020 - synapse.koreamed.org

ABSTRACT Novel coronavirus (SARS-CoV-2) is found to cause a large outbreak started from Wuhan since December 2019 in China and SARS-CoV-2 infections have been reported with epidemiological linkage to China in 25 countries until now. We isolated SARS …

 Cited by 124 Related articles 

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Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells

S Matsuyama, N Nao, K Shirato… - Proceedings of the …, 2020 - National Acad Sciences

… Physiology; Plant Biology; Population Biology; Psychological and Cognitive Sciences; Sustainability Science; Systems Biology. Brief Report. Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells. Shutoku Matsuyama …

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Coronaviruses and SARS-CoV-2: a brief overview

S Ludwig, A Zarbock - Anesthesia and analgesia, 2020 - ncbi.nlm.nih.gov

… like appearance under the electron microscope, which has led to the name corona (Latin: garland … On the origin and continuing evolution of SARS-CoV-2. Natl Sci Rev … Molecular and serological investigation of 2019-nCoV infected patients: implication of multiple shedding routes …

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Collection of SARS-CoV-2 virus from the air of a clinic within a university student health care center and analyses of the viral genomic sequence

JA Lednicky, SN Shankar, MA Elbadry… - Aerosol and air …, 2020 - ncbi.nlm.nih.gov

… Transmission of 2019-nCoV Infection from an Asymptomatic Contact in Germany … Transmission Potential of SARS-CoV-2 in Viral Shedding Observed at the University of … Modes of Transmission of Virus Causing COVID-19: Implications for Ipc Precaution Recommendations, In …

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Infection of dogs with SARS-CoV-2

THC Sit, CJ Brackman, SM Ip, KWS Tam, PYT Law… - Nature, 2020 - nature.com

… CoV-2) was first detected in Wuhan in December 2019 and caused coronavirus disease 2019 … that 2 out of 15 dogs from households with confirmed human cases of COVID-19 in Hong … A 2.5-year-old male German shepherd was positive for SARS-CoV-2 RNA on two occasions …

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Identification of coronavirus isolated from a patient in Korea with COVID-19

JM Kim, YS Chung, HJ Jo, NJ Lee, MS Kim… - Osong public health …, 2020 - ncbi.nlm.nih.gov

… Currently, the diagnosis of COVID-19 is based on gene detection via real-time RT-PCR … SARS and MERS: Recent insights into emerging coronaviruses … Full-genome evolutionary analysis of the novel corona virus (2019-nCoV) rejects the hypothesis of emergence as a result of a …

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Infection and rapid transmission of SARS-CoV-2 in ferrets

YI Kim, SG Kim, SM Kim, EH Kim, SJ Park, KM Yu… - Cell host & …, 2020 - Elsevier

… a newly emerged strain of betacoronavirus, temporarily named 2019 novel coronavirus (2019-nCoV) and later … These data indicate that the efficient establishment of COVID-19 clinical features in ferrets … To assess the replication of SARS-CoV-2 in ferret organs, an additional 12 …

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Presence and infectivity of SARS-CoV-2 virus in wastewaters and rivers

SG Rimoldi, F Stefani, A Gigantiello, S Polesello… - Science of the Total …, 2020 - Elsevier

… and 0.67% respectively) respect to the rest of Italy (0.40%) (https://github.com/pcm-dpc/COVID- 19/blob/master … Primers set was designed by manufacturer for both universal detection of SARS-like coronaviruses as well as specific detection of six 2019-nCoV strains …

 Cited by 115 Related articles 

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