PEMF stimulating muscle cells to fight cancer "
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Posted On: 03/06/2024 5:27:54 PM
PEMF stimulating muscle cells to fight cancer
"This study was undertaken to investigate the potential of PEMF therapy to serve as a non-invasive manner to efficiently mobilize the secretome of muscle for soluble biomarker discovery and anticancer validation."
5. Conclusions
Muscle cells briefly exposed to PEMFs produced a conditioned media capable of inhibiting breast cancer cell growth, migration, and invasion in vitro and ex vivo, whereas conditioned media harvested from unexposed muscle cells, representing the constitutive release of secretome from unexposed muscle cells, was less effective. Blood sera collected from PEMF-exposed or exercised mice also inhibited the proliferation, migration, and invasiveness of breast cancer cells, providing physiological relevance to the in vitro results. A secretome preconditioning methodology was designed to enhance secretome anticancer potency. Importantly, the anticancer potency of the constitutively released secretome from unexposed muscle was also enhanced. Myogenic differentiation was also enhanced with pCM preconditioning, confirming an adaptive response to secretome induction akin to exercise training in animals. Via this drug-free system, secretome production and release are both effectively enhanced with minimal non-invasive intervention. Given the demonstrated ability of PEMF exposure to induce secretome release and its ease of application (10 min exposures) in both cells (once) and animals (weekly), magnetic paradigms may offer a unique opportunity for soluble biomarker discovery and clinical exploitation. Aside from the immediate clinical implications of muscle secretome magnetic induction, this study paves the way for the development of drug-free magnetic platforms for the discovery and characterization of novel liquid biomarkers with relevance to human cancer; HTRA1 was highlighted as a notable example.
https://www.mdpi.com/2073-4409/13/5/460
"This study was undertaken to investigate the potential of PEMF therapy to serve as a non-invasive manner to efficiently mobilize the secretome of muscle for soluble biomarker discovery and anticancer validation."
5. Conclusions
Muscle cells briefly exposed to PEMFs produced a conditioned media capable of inhibiting breast cancer cell growth, migration, and invasion in vitro and ex vivo, whereas conditioned media harvested from unexposed muscle cells, representing the constitutive release of secretome from unexposed muscle cells, was less effective. Blood sera collected from PEMF-exposed or exercised mice also inhibited the proliferation, migration, and invasiveness of breast cancer cells, providing physiological relevance to the in vitro results. A secretome preconditioning methodology was designed to enhance secretome anticancer potency. Importantly, the anticancer potency of the constitutively released secretome from unexposed muscle was also enhanced. Myogenic differentiation was also enhanced with pCM preconditioning, confirming an adaptive response to secretome induction akin to exercise training in animals. Via this drug-free system, secretome production and release are both effectively enhanced with minimal non-invasive intervention. Given the demonstrated ability of PEMF exposure to induce secretome release and its ease of application (10 min exposures) in both cells (once) and animals (weekly), magnetic paradigms may offer a unique opportunity for soluble biomarker discovery and clinical exploitation. Aside from the immediate clinical implications of muscle secretome magnetic induction, this study paves the way for the development of drug-free magnetic platforms for the discovery and characterization of novel liquid biomarkers with relevance to human cancer; HTRA1 was highlighted as a notable example.
https://www.mdpi.com/2073-4409/13/5/460
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