Brilacidin, currently being used by Degrado in fur
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Posted On: 09/17/2021 2:06:54 AM
Brilacidin, currently being used by Degrado in further research. This is minor and fragmentary news. But together with other findings the big picture is being brought into focus.
Note: Brilacidin is an antibiotic that works by disrupting bacterial cell membranes, mimicking defensins that play a role in innate immunity.[8][9] Several mimics of antimicrobial peptides, both peptides and non-peptides, have been studied, but none have overcome difficulties to reach the market.
In a recent arrival, Degrado is found using BRILACIDIN in a lab on other than just COVID-19.
Extract from an article dealing with cell structure issues and effects notably:
pcxP, pcxA Etc. Etc.— read the link below for extreme details
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Measuring activity of CpxA, BaeS: HK constructs were cloned into the MCS of pTrc99a plasmid, and the associated fluorescent reporter gene was cloned downstream. For the CpxA reporter plasmid, the response regulator CpxR, was also cloned into the MCS and transformed
Iinto AFS51 strain (DcpxADpta::Kan pcpxP::GFP) by heat shock transformation. For BaeS, the response regulator BaeR, was cloned into an additional plasmid, pSEVA331 under an IPTG inducible promoter and both plasmids were transformed into a ∆baeS∆cpxA double KO strain by
heat shock transformation. Cultures were started by diluting overnights 200-500 folds into fresh LB + 50 μg/mL AMP media and allowed to grow to mid-log phase (OD600 = 0.4 – 0.6) before
analysis by flow cytometry.
The responsiveness of cpxP reporter was confirmed by treating log-phase cultures with 2 μg/mL BRILACIDIN for 1.5 hours before analysis.
Expression of HKs was confirmed by western analysis using the c-terminal 6x His-tag for quantification.
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Why do we care? Brilacidin is being accepted at an international level by leading research scientist.
HERE IS THE LINK TO THE ORIGINAL ARTICAL
https://www.biorxiv.org/content/10.1101/2021....1.full.pdf
Note: Brilacidin is an antibiotic that works by disrupting bacterial cell membranes, mimicking defensins that play a role in innate immunity.[8][9] Several mimics of antimicrobial peptides, both peptides and non-peptides, have been studied, but none have overcome difficulties to reach the market.
In a recent arrival, Degrado is found using BRILACIDIN in a lab on other than just COVID-19.
Extract from an article dealing with cell structure issues and effects notably:
pcxP, pcxA Etc. Etc.— read the link below for extreme details
**********
Measuring activity of CpxA, BaeS: HK constructs were cloned into the MCS of pTrc99a plasmid, and the associated fluorescent reporter gene was cloned downstream. For the CpxA reporter plasmid, the response regulator CpxR, was also cloned into the MCS and transformed
Iinto AFS51 strain (DcpxADpta::Kan pcpxP::GFP) by heat shock transformation. For BaeS, the response regulator BaeR, was cloned into an additional plasmid, pSEVA331 under an IPTG inducible promoter and both plasmids were transformed into a ∆baeS∆cpxA double KO strain by
heat shock transformation. Cultures were started by diluting overnights 200-500 folds into fresh LB + 50 μg/mL AMP media and allowed to grow to mid-log phase (OD600 = 0.4 – 0.6) before
analysis by flow cytometry.
The responsiveness of cpxP reporter was confirmed by treating log-phase cultures with 2 μg/mL BRILACIDIN for 1.5 hours before analysis.
Expression of HKs was confirmed by western analysis using the c-terminal 6x His-tag for quantification.
*********
Why do we care? Brilacidin is being accepted at an international level by leading research scientist.
HERE IS THE LINK TO THE ORIGINAL ARTICAL
https://www.biorxiv.org/content/10.1101/2021....1.full.pdf
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Ronald A Phillips