https://www.news-medical.net/news/20210317/Quantum
Post# of 22454
(Total Views: 650)
Posted On: 03/17/2021 10:08:09 PM
https://www.news-medical.net/news/20210317/Qu...tform.aspx
News-Medical
Become a Member
Search...
Search
Menu
4
Quantum dot-based affinity biosensors in microfluidic systems offer an alternative SARS-CoV-2 diagnostics platform
Download PDF Copy
Dr. Sanchari Sinha Dutta, Ph.D.
By Dr. Sanchari Sinha Dutta, Ph.D.Mar 17 2021
In a new study published in Emergent Materials, researchers show how microfluidic systems integrated with quantum dot-based affinity biosensors can serve as a valuable platform to rapidly and accurately diagnose severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection.
Study: Affinity biosensors developed with quantum dots in microfluidic systems. Image Credit: NIAID / Flickr
Study: Affinity biosensors developed with quantum dots in microfluidic systems. Image Credit: NIAID / Flickr
What are quantum dots?
Quantum dots (QD) are nanoscale semiconductor crystals (size: 2-10 nm) with unique optical and electronic characteristics. Such nanomaterials are widely used in biosensing applications for biomedical and bioanalytical purposes. Unlike organic dyes and protein-based fluorophores, QDs have a wide absorption spectrum and narrow emission bands, which make them very suitable for cell imaging and biosensor applications. Specifically, QDs can be used to trace multiple biological targets simultaneously with simple instrumentations. Other properties of QDs that make them superior to organic dyes include higher quantum efficiency and molar absorption coefficient. Because of the semiconductor properties, QDs can also be used in electrochemical applications.
Quantum dots in biosensing
Biosensors are an analytical device that combines a biomolecule to detect an analyte and a physicochemical component to produce a measurable signal. In affinity and catalytic biosensor applications, QDs are used to develop optical and electrochemical transduction systems. In affinity biosensors, affinity-based biomolecules are immobilized on the transducer to reversibly detect analytes with high affinity and selectivity. The main principle is the formation of a stable and selective interaction between the biomolecule and analyte, which in turn generates a response signal by the transducer. The most commonly used biomolecules in affinity biosensors are antigen-antibody, oligonucleotides, aptamers, etc.
One of the recent advancements in biosensor applications is the integration of biosensing mechanisms in the microfluid system. In this approach, ODs are conjugated with the biomolecule via covalent or non-covalent binding approaches. In other words, microfluidic systems serve as a platform wherein QDs are integrated for biosensing applications. The main advantage of a microfluidic system is mass transfer in short diffusion distance. Similarly, the main benefit of QDs is a large surface area/volume ratio. By combining the benefits of QDs with the microfluidic system, it is possible to increase the sensitivity and reduce the response time of optical biosensors. Thus, a QD-based biosensor integrated with a microfluidic system can serve as an important tool to rapidly and accurately diagnose SARS-CoV-2 infection.
QD-based biosensor integrated with microfluidic system
Related Stories
Data from Israel’s vaccination program suggests Pizer-BioNTech COVID-19 vaccine induces strong host antibody response
Novavax COVID-19 vaccine shows efficacy against South African SARS-CoV-2 variant
Study demonstrates long-term neuropsychological consequences following SARS-CoV-2 infection
In the microfluidic platform, polydimethylsiloxane-based microchannels are used. Polydimethylsiloxane, a silicon-based organic polymer, is highly elastic, non-toxic, biocompatible, and optically transparent. The channel surface is modified in microsystems to develop optical and electrochemical biosensors. In addition, the sensor region can be developed with magnetic particles or polystyrene microspheres (carrier solid supports) by creating an active detection zone in the microchannels. In a microfluid system without carrier solid support, immobilization techniques are used to create a detection zone on the microchannel surface.
Potential applications
QD-based biosensors integrated with a microfluidic system can be used for real-time, lab-quality diagnostic purposes. Such portable designs are particularly helpful for rapid and accurate detection of various biomedical conditions. The rapid adaptability of fluidic detection systems to QD-based biosensor applications encourages faster development of new detectors for coronavirus disease 2019 (COVID-19) diagnosis.
The QD-based optical biosensor has been developed in the microfluidic system to detect various viral genotypes, cancer biomarkers, and pathogens (coronavirus, human immunodeficiency virus, and Zika virus). Recently, polymerase chain reaction (PCR) for SARS-CoV-2 has been conducted in an electrochemical microfluidic device for the detection of amplified nucleic acids on a silicon-based transducer. Similarly, an optical microfluidic device has been developed to detect anti-SARS-CoV-2 antibodies using surface plasmon resonance transduction method. Using this method, SARS-CoV-2 spike protein-specific antibodies can be detected in diluted human plasma samples within 30 min. In addition, a fluorescence-based microfluidic detection system has been developed to simultaneously detect IgG/IgM antibodies and antigens of SARS-CoV-2 within 15 min.
In addition, QDs can be used as donor molecules in fluorescence resonance energy transfer (FRET) assay. By integrating QD-based FRET with affinity biosensing, it is possible to detect proteins, nucleic acids, and small molecules.
Journal reference:
Sahin S. et al. (2021). Affinity biosensors developed with quantum dots in microfluidic systems. Emergent Materials https://doi.org/10.1007/s42247-021-00195-5, https://link.springer.com/article/10.1007/s42247-021-00195-5
Be the first to rate this article
Posted in: Device / Technology News | Medical Science News | Medical Research News | Disease/Infection News | Healthcare News
Tags: Analyte, Antibodies, Antibody, Antigen, Aptamers, Assay, Biosensor, Cancer, Cell, Cell Imaging, Coronavirus, Coronavirus Disease COVID-19, Diagnostic, Diagnostics, Fluorescence, FRET, Imaging, Immunodeficiency, Oligonucleotides, Polymerase, Polymerase Chain Reaction, Protein, Quantum Dots, Quantum Efficiency, Respiratory, SARS, SARS-CoV-2, Severe Acute Respiratory, Severe Acute Respiratory Syndrome, Spike Protein, Syndrome, Virus, Zika Virus
Comments (0)
Dr. Sanchari Sinha Dutta
Written by
Dr. Sanchari Sinha Dutta
Dr. Sanchari Sinha Dutta is a science communicator who believes in spreading the power of science in every corner of the world. She has a Bachelor of Science (B.Sc.) degree and a Master's of Science (M.Sc.) in biology and human physiology. Following her Master's degree, Sanchari went on to study a Ph.D. in human physiology. She has authored more than 10 original research articles, all of which have been published in world renowned international journals.
Download PDF Copy
Read in:English
Citations
Suggested Reading
Pfizer COVID-19 vaccine elicits antibodies in blood and breastmilk simultaneously, finds study
Pfizer COVID-19 vaccine elicits antibodies in blood and breastmilk simultaneously, finds study
UK variant of SARS-CoV-2 could be more deadly than previous strains
UK variant of SARS-CoV-2 could be more deadly than previous strains
Newly-discovered bat coronavirus 94.5% identical to SARS-CoV-2
Newly-discovered bat coronavirus 94.5% identical to SARS-CoV-2
New evidence of SARS-CoV-2 spreading on planes
New evidence of SARS-CoV-2 spreading on planes
Study confirms B.1.1.7 variant of SARS-CoV-2 is more transmissible
Study confirms B.1.1.7 variant of SARS-CoV-2 is more transmissible
How likely is SARS-CoV-2 reinfection?
How likely is SARS-CoV-2 reinfection?
Results from phase 2 trial of Novavax recombinant nanoparticle SARS-CoV-2 vaccine
Results from phase 2 trial of Novavax recombinant nanoparticle SARS-CoV-2 vaccine
Pfizer SARS-CoV-2 vaccine effective from day 11 post 1st vaccination
Pfizer SARS-CoV-2 vaccine effective from day 11 post 1st vaccination
Comments
The opinions expressed here are the views of the writer and do not necessarily reflect the views and opinions of News Medical.
Post a new comment
Login
Quirky Comment Title (optional)
Post
Trending
Stories
Latest
Interviews
Top Health
Articles
Cannabis compound inhibits SARS-CoV-2 replication in human lung cells
Cannabis compound inhibits SARS-CoV-2 replication in human lung cells
Newly-discovered bat coronavirus 94.5% identical to SARS-CoV-2
Newly-discovered bat coronavirus 94.5% identical to SARS-CoV-2
Sharp declines in opioid production costs could reduce the price per dose
Sharp declines in opioid production costs could reduce the price per dose
Music-based interventions can improve stroke, Parkinson's disease
Music-based interventions can improve stroke, Parkinson's disease
Danish study on the efficacy of Pfizer/BioNTech SARS-CoV-2 vaccine after first and second dose
Danish study on the efficacy of Pfizer/BioNTech SARS-CoV-2 vaccine after first and second dose
Latest News
Scientists identify address codes in odorant receptor proteins
Scientists identify address codes in odorant receptor proteins
Digital communication can bridge the racial disparity gap in cervical cancer care
Digital communication can bridge the racial disparity gap in cervical cancer care
Older and non-White people less likely to be captured by mobility data used in response to COVID-19
Older and non-White people less likely to be captured by mobility data used in response to COVID-19
Lab-created heart valves implanted in young lambs can grow with the animal
Lab-created heart valves implanted in young lambs can grow with the animal
UTSW researchers identify the structure of a key cell-surface protein
UTSW researchers identify the structure of a key cell-surface protein
Newsletters you may be interested in
Clinical and Lab DiagnosticsClinical and Lab Diagnostics
(Subscribe or Preview)
AutismAutism
(Subscribe or Preview)
HIV/AIDSHIV/AIDS
(Subscribe or Preview)
See all Newsletters »
HONCode Logo certificate HONCode Certified Seal TRUSTe
Medical Links
Medical Home
COVID-19
News
Health A-Z
White Papers
Thought Leaders
Insights
MediKnowledge Series
Health & Personal Care
Medical Devices
Drugs
Life Sciences Links
Life Sciences Home
News
Lab Instruments & Equipment
Life Sciences A-Z
White Papers
Thought Leaders
Insights
Webinars
Other Useful Links
About
Meet the Team
Search
Newsletters
Sitemap
Advertise
Contact
Update Your Privacy Preferences
Terms & Conditions
Privacy & Cookie Policy
News-Medical
Facebook Twitter
News-Medical.Net provides this medical information service in accordance with these terms and conditions. Please note that medical information found on this website is designed to support, not to replace the relationship between patient and physician/doctor and the medical advice they may provide.
This website is certified by Health On the Net Foundation. Click to verify.
This site complies with the HONcode standard for trustworthy health information: verify here.
AZoNetwork
News-Medical.net - An AZoNetwork Site
Owned and operated by AZoNetwork, © 2000-2021
We use cookies to enhance your experience. By continuing to browse this site you agree to our use of cookies. More info.
Accept Cookie Settings
News-Medical
Become a Member
Search...
Search
Menu
4
Quantum dot-based affinity biosensors in microfluidic systems offer an alternative SARS-CoV-2 diagnostics platform
Download PDF Copy
Dr. Sanchari Sinha Dutta, Ph.D.
By Dr. Sanchari Sinha Dutta, Ph.D.Mar 17 2021
In a new study published in Emergent Materials, researchers show how microfluidic systems integrated with quantum dot-based affinity biosensors can serve as a valuable platform to rapidly and accurately diagnose severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection.
Study: Affinity biosensors developed with quantum dots in microfluidic systems. Image Credit: NIAID / Flickr
Study: Affinity biosensors developed with quantum dots in microfluidic systems. Image Credit: NIAID / Flickr
What are quantum dots?
Quantum dots (QD) are nanoscale semiconductor crystals (size: 2-10 nm) with unique optical and electronic characteristics. Such nanomaterials are widely used in biosensing applications for biomedical and bioanalytical purposes. Unlike organic dyes and protein-based fluorophores, QDs have a wide absorption spectrum and narrow emission bands, which make them very suitable for cell imaging and biosensor applications. Specifically, QDs can be used to trace multiple biological targets simultaneously with simple instrumentations. Other properties of QDs that make them superior to organic dyes include higher quantum efficiency and molar absorption coefficient. Because of the semiconductor properties, QDs can also be used in electrochemical applications.
Quantum dots in biosensing
Biosensors are an analytical device that combines a biomolecule to detect an analyte and a physicochemical component to produce a measurable signal. In affinity and catalytic biosensor applications, QDs are used to develop optical and electrochemical transduction systems. In affinity biosensors, affinity-based biomolecules are immobilized on the transducer to reversibly detect analytes with high affinity and selectivity. The main principle is the formation of a stable and selective interaction between the biomolecule and analyte, which in turn generates a response signal by the transducer. The most commonly used biomolecules in affinity biosensors are antigen-antibody, oligonucleotides, aptamers, etc.
One of the recent advancements in biosensor applications is the integration of biosensing mechanisms in the microfluid system. In this approach, ODs are conjugated with the biomolecule via covalent or non-covalent binding approaches. In other words, microfluidic systems serve as a platform wherein QDs are integrated for biosensing applications. The main advantage of a microfluidic system is mass transfer in short diffusion distance. Similarly, the main benefit of QDs is a large surface area/volume ratio. By combining the benefits of QDs with the microfluidic system, it is possible to increase the sensitivity and reduce the response time of optical biosensors. Thus, a QD-based biosensor integrated with a microfluidic system can serve as an important tool to rapidly and accurately diagnose SARS-CoV-2 infection.
QD-based biosensor integrated with microfluidic system
Related Stories
Data from Israel’s vaccination program suggests Pizer-BioNTech COVID-19 vaccine induces strong host antibody response
Novavax COVID-19 vaccine shows efficacy against South African SARS-CoV-2 variant
Study demonstrates long-term neuropsychological consequences following SARS-CoV-2 infection
In the microfluidic platform, polydimethylsiloxane-based microchannels are used. Polydimethylsiloxane, a silicon-based organic polymer, is highly elastic, non-toxic, biocompatible, and optically transparent. The channel surface is modified in microsystems to develop optical and electrochemical biosensors. In addition, the sensor region can be developed with magnetic particles or polystyrene microspheres (carrier solid supports) by creating an active detection zone in the microchannels. In a microfluid system without carrier solid support, immobilization techniques are used to create a detection zone on the microchannel surface.
Potential applications
QD-based biosensors integrated with a microfluidic system can be used for real-time, lab-quality diagnostic purposes. Such portable designs are particularly helpful for rapid and accurate detection of various biomedical conditions. The rapid adaptability of fluidic detection systems to QD-based biosensor applications encourages faster development of new detectors for coronavirus disease 2019 (COVID-19) diagnosis.
The QD-based optical biosensor has been developed in the microfluidic system to detect various viral genotypes, cancer biomarkers, and pathogens (coronavirus, human immunodeficiency virus, and Zika virus). Recently, polymerase chain reaction (PCR) for SARS-CoV-2 has been conducted in an electrochemical microfluidic device for the detection of amplified nucleic acids on a silicon-based transducer. Similarly, an optical microfluidic device has been developed to detect anti-SARS-CoV-2 antibodies using surface plasmon resonance transduction method. Using this method, SARS-CoV-2 spike protein-specific antibodies can be detected in diluted human plasma samples within 30 min. In addition, a fluorescence-based microfluidic detection system has been developed to simultaneously detect IgG/IgM antibodies and antigens of SARS-CoV-2 within 15 min.
In addition, QDs can be used as donor molecules in fluorescence resonance energy transfer (FRET) assay. By integrating QD-based FRET with affinity biosensing, it is possible to detect proteins, nucleic acids, and small molecules.
Journal reference:
Sahin S. et al. (2021). Affinity biosensors developed with quantum dots in microfluidic systems. Emergent Materials https://doi.org/10.1007/s42247-021-00195-5, https://link.springer.com/article/10.1007/s42247-021-00195-5
Be the first to rate this article
Posted in: Device / Technology News | Medical Science News | Medical Research News | Disease/Infection News | Healthcare News
Tags: Analyte, Antibodies, Antibody, Antigen, Aptamers, Assay, Biosensor, Cancer, Cell, Cell Imaging, Coronavirus, Coronavirus Disease COVID-19, Diagnostic, Diagnostics, Fluorescence, FRET, Imaging, Immunodeficiency, Oligonucleotides, Polymerase, Polymerase Chain Reaction, Protein, Quantum Dots, Quantum Efficiency, Respiratory, SARS, SARS-CoV-2, Severe Acute Respiratory, Severe Acute Respiratory Syndrome, Spike Protein, Syndrome, Virus, Zika Virus
Comments (0)
Dr. Sanchari Sinha Dutta
Written by
Dr. Sanchari Sinha Dutta
Dr. Sanchari Sinha Dutta is a science communicator who believes in spreading the power of science in every corner of the world. She has a Bachelor of Science (B.Sc.) degree and a Master's of Science (M.Sc.) in biology and human physiology. Following her Master's degree, Sanchari went on to study a Ph.D. in human physiology. She has authored more than 10 original research articles, all of which have been published in world renowned international journals.
Download PDF Copy
Read in:English
Citations
Suggested Reading
Pfizer COVID-19 vaccine elicits antibodies in blood and breastmilk simultaneously, finds study
Pfizer COVID-19 vaccine elicits antibodies in blood and breastmilk simultaneously, finds study
UK variant of SARS-CoV-2 could be more deadly than previous strains
UK variant of SARS-CoV-2 could be more deadly than previous strains
Newly-discovered bat coronavirus 94.5% identical to SARS-CoV-2
Newly-discovered bat coronavirus 94.5% identical to SARS-CoV-2
New evidence of SARS-CoV-2 spreading on planes
New evidence of SARS-CoV-2 spreading on planes
Study confirms B.1.1.7 variant of SARS-CoV-2 is more transmissible
Study confirms B.1.1.7 variant of SARS-CoV-2 is more transmissible
How likely is SARS-CoV-2 reinfection?
How likely is SARS-CoV-2 reinfection?
Results from phase 2 trial of Novavax recombinant nanoparticle SARS-CoV-2 vaccine
Results from phase 2 trial of Novavax recombinant nanoparticle SARS-CoV-2 vaccine
Pfizer SARS-CoV-2 vaccine effective from day 11 post 1st vaccination
Pfizer SARS-CoV-2 vaccine effective from day 11 post 1st vaccination
Comments
The opinions expressed here are the views of the writer and do not necessarily reflect the views and opinions of News Medical.
Post a new comment
Login
Quirky Comment Title (optional)
Post
Trending
Stories
Latest
Interviews
Top Health
Articles
Cannabis compound inhibits SARS-CoV-2 replication in human lung cells
Cannabis compound inhibits SARS-CoV-2 replication in human lung cells
Newly-discovered bat coronavirus 94.5% identical to SARS-CoV-2
Newly-discovered bat coronavirus 94.5% identical to SARS-CoV-2
Sharp declines in opioid production costs could reduce the price per dose
Sharp declines in opioid production costs could reduce the price per dose
Music-based interventions can improve stroke, Parkinson's disease
Music-based interventions can improve stroke, Parkinson's disease
Danish study on the efficacy of Pfizer/BioNTech SARS-CoV-2 vaccine after first and second dose
Danish study on the efficacy of Pfizer/BioNTech SARS-CoV-2 vaccine after first and second dose
Latest News
Scientists identify address codes in odorant receptor proteins
Scientists identify address codes in odorant receptor proteins
Digital communication can bridge the racial disparity gap in cervical cancer care
Digital communication can bridge the racial disparity gap in cervical cancer care
Older and non-White people less likely to be captured by mobility data used in response to COVID-19
Older and non-White people less likely to be captured by mobility data used in response to COVID-19
Lab-created heart valves implanted in young lambs can grow with the animal
Lab-created heart valves implanted in young lambs can grow with the animal
UTSW researchers identify the structure of a key cell-surface protein
UTSW researchers identify the structure of a key cell-surface protein
Newsletters you may be interested in
Clinical and Lab DiagnosticsClinical and Lab Diagnostics
(Subscribe or Preview)
AutismAutism
(Subscribe or Preview)
HIV/AIDSHIV/AIDS
(Subscribe or Preview)
See all Newsletters »
HONCode Logo certificate HONCode Certified Seal TRUSTe
Medical Links
Medical Home
COVID-19
News
Health A-Z
White Papers
Thought Leaders
Insights
MediKnowledge Series
Health & Personal Care
Medical Devices
Drugs
Life Sciences Links
Life Sciences Home
News
Lab Instruments & Equipment
Life Sciences A-Z
White Papers
Thought Leaders
Insights
Webinars
Other Useful Links
About
Meet the Team
Search
Newsletters
Sitemap
Advertise
Contact
Update Your Privacy Preferences
Terms & Conditions
Privacy & Cookie Policy
News-Medical
Facebook Twitter
News-Medical.Net provides this medical information service in accordance with these terms and conditions. Please note that medical information found on this website is designed to support, not to replace the relationship between patient and physician/doctor and the medical advice they may provide.
This website is certified by Health On the Net Foundation. Click to verify.
This site complies with the HONcode standard for trustworthy health information: verify here.
AZoNetwork
News-Medical.net - An AZoNetwork Site
Owned and operated by AZoNetwork, © 2000-2021
We use cookies to enhance your experience. By continuing to browse this site you agree to our use of cookies. More info.
Accept Cookie Settings
(2)
(0)Quantum Materials Corp. (QTMM) Stock Research Links
Scroll down for more posts ▼