As I mentioned in the post you're reply to, the 25kL
As I mentioned in the post you're reply to, the 25kL bioreactor is likely just the size (max capacity) of the bioreactor, not the working volume of our process.
For example, I have worked on a lot of processes that use 18kL bioreactors. The final working volume could range from 11kL to 16kL and could be 15435 L for instance.
They are operated like this for a WIDE range of reasons some include mixing and gassing capabilities of the tank system in relations to the cell culture's rate of consumption and metabolic profile.
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