This old article shows Ccr5 density, huge differen
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Posted On: 08/05/2019 7:47:46 PM
This old article shows Ccr5 density, huge difference, 60% more receptors mean plus wider deviation, in A/A compared to A/G, GG. So the A/A group is probably the group causing the failures.
I’m trying to find out percent of A/A, https://www.snpedia.com/index.php/CCR5
rs1799987, CCR5 59029G, also known as -2459
CCR5 promoter polymorphism determines macrophage CCR5 density and magnitude of HIV-1 propagation in vitro
https://www.sciencedirect.com/science/article...1603001475
Abstract
The common CCR5 promoter polymorphism at position 2459 (A/G) has been associated with differences in the rate of progressionto AIDS, where HIV-1-infected individuals with the CCR52459 G/G genotype exhibit slower disease progression than those with the A/Agenotype. Mechanisms underlying the relationship between these polymorphisms and disease progression are not known. Here through invitro infection of peripheral blood mononuclear cells obtained from healthy Caucasian blood donors with macrophage-tropic HIV-1 isolateswe observed low, medium, and high viral propagation in association with G/G, A/G, and A/A promoter genotypes, respectively. Flowcytometric analysis of unstimulated CD14monocytes from these same donors revealed a similar hierarchy of CCR5 receptor density inassociation with promoter genotypes. Finally, PBMC from persons with the G/G promoter polymorphism produced higher levels of-chemokines after in vitro stimulation. Thus, the CCR52459 (A/G) promoter polymorphism determines CCR5 expression and predictsthe magnitude of HIV-1 propagation in vitro. These findings may provide important insight regarding the regulation of mechanisms thatinfluence the rate of HIV-1 propagation and progression to AIDS
Results
Since CCR5 promoter polymorphisms at 2459 were previously associated with magnitude of linked reportergene activity [11], we asked if this SNP influenced thedensity of CCR5 on CD4 mononuclear cell subsets. By flow cytometric analyses, we observed that the density ofCCR5 was lower on unstimulated CD14 monocytes fromhealthy individuals with the 2459 G/G and A/G (10,378 +- 4,289 receptors/cell) genotypes compared to A/A (16,411 +- 7,334 receptors/cell) genotypes (Fig. 1;P<0.05 two-sidedttest). In contrast, the densities of CCR5 on total resting CD4 T cells and CD4 T cells stimulated with PHA andIL-2 were comparable in persons with the G/G, A/G, and A/A genotype
I’m trying to find out percent of A/A, https://www.snpedia.com/index.php/CCR5
rs1799987, CCR5 59029G, also known as -2459
CCR5 promoter polymorphism determines macrophage CCR5 density and magnitude of HIV-1 propagation in vitro
https://www.sciencedirect.com/science/article...1603001475
Abstract
The common CCR5 promoter polymorphism at position 2459 (A/G) has been associated with differences in the rate of progressionto AIDS, where HIV-1-infected individuals with the CCR52459 G/G genotype exhibit slower disease progression than those with the A/Agenotype. Mechanisms underlying the relationship between these polymorphisms and disease progression are not known. Here through invitro infection of peripheral blood mononuclear cells obtained from healthy Caucasian blood donors with macrophage-tropic HIV-1 isolateswe observed low, medium, and high viral propagation in association with G/G, A/G, and A/A promoter genotypes, respectively. Flowcytometric analysis of unstimulated CD14monocytes from these same donors revealed a similar hierarchy of CCR5 receptor density inassociation with promoter genotypes. Finally, PBMC from persons with the G/G promoter polymorphism produced higher levels of-chemokines after in vitro stimulation. Thus, the CCR52459 (A/G) promoter polymorphism determines CCR5 expression and predictsthe magnitude of HIV-1 propagation in vitro. These findings may provide important insight regarding the regulation of mechanisms thatinfluence the rate of HIV-1 propagation and progression to AIDS
Results
Since CCR5 promoter polymorphisms at 2459 were previously associated with magnitude of linked reportergene activity [11], we asked if this SNP influenced thedensity of CCR5 on CD4 mononuclear cell subsets. By flow cytometric analyses, we observed that the density ofCCR5 was lower on unstimulated CD14 monocytes fromhealthy individuals with the 2459 G/G and A/G (10,378 +- 4,289 receptors/cell) genotypes compared to A/A (16,411 +- 7,334 receptors/cell) genotypes (Fig. 1;P<0.05 two-sidedttest). In contrast, the densities of CCR5 on total resting CD4 T cells and CD4 T cells stimulated with PHA andIL-2 were comparable in persons with the G/G, A/G, and A/A genotype
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