The song was featured during a scene in the 1978 film The Deer Hunter by director Michael Cimino, in which the character Nick (Christopher Walken) - having left the military hospital - is visiting a strip club in Saigon as the girls gyrate to "Midnight Train to Georgia".
"Episode 210" of the NBC sitcom 30 Rock features an extensive parody of the song, punctuated by a cameo from Knight herself. The song was also featured in the episode "The Choice" of House, and the episode "Swimming Pools ... Movie Stars" of Will & Grace. Ben Stiller, Jack Black and Robert Downey Jr. reenacted The Pips' dance moves from a live performance of the song for the American Idol finale.
NBC invited Gladys Knight to the season two finale of Las Vegas. She sang "Best Thing That Ever Happened to Me" and "Midnight Train To Georgia".
In the movie He Was a Quiet Man, Elisha Cuthbert and Christian Slater do a gig of this song at a restaurant when Venessa (Cuthbert) gets released to home care from the hospital. Bob McConnel (Slater) does the Pips.
The song was also mentioned in "The Ice of Boston", a song on The Dismemberment Plan's 1997 album The Dismemberment Plan Is Terrified.
In 2013, the song is mentioned and later sung by cast members of the ABC sitcom Modern Family in the episode entitled, The Late Show.
Featured in the 2014 film, The Equalizer, as a nod to a Gladys Knight & the Pips reference made early in the film.
It was also used during an episode of Scandal in 2015.