THE "REAL STORY" BEHIND THE NMU~MIT1000 COLLABORAT
Post# of 2022
Posted On: 09/07/2014 10:51:15 AM
THE "REAL STORY" BEHIND THE NMU~MIT1000 COLLABORATION!!!
Quote:
Nucleic acid amplification tests, such as the polymerase chain reaction (PCR), can be used to detect the mecA gene, (IT'S)is the most common gene that mediates oxacillin resistance in staphylococci.
However, mecA PCR tests will not detect novel resistance mechanisms such as mecC or uncommon phenotypes such as borderline-resistant oxacillin resistance. ~ CDC
http://www.cdc.gov/MRSA/lab/index.html
Quote:
The time taken for diagnosing MRSA by conventional methods is 48-72 h, which is more as compared to PCR which takes 18-24 h.
Conclusion: This study recommends advocating PCR for mecA gene on a regular basis for detecting methicillin resistance in S. aureus isolates isolated from sterile body fluids or from special units such as intensive care units.
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Laboratory diagnosis and susceptibility testing are crucial steps in treating, controlling, and preventing MRSA infections. Discrepancies in detection have an adverse effect on patient management, thereby highlighting the importance of accuracy in detection. Hence, methods used to detect MRSA in clinical samples should have high sensitivity and specificity and, most importantly, the result should be available within a short time. Various methods have evolved for rapid detection of methicillin-resistant staphylococci, but the optimal method for the detection remains controversial. The most commonly used method in the laboratories is culture and antibiotic sensitivity test (AST) [oxacillin disk diffusion (ODD)]. Other methods available for diagnosing MRSA include mannitol salt agar (MSA) with oxacillin (agar screening method), minimum inhibitory concentration (MIC) tests, agar dilution tests etc., All these tests are the conventional phenotypic methods of MRSA identification. [12],[13] Genotypic (molecular) method is the polymerase chain reaction (PCR) based method for detecting mecA gene, which remains the "gold standard" for diagnosing MRSA. [8],[13],[14]
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Our study concludes that the commonly used phenotypic tests are not completely reliable for the detection of methicillin resistance in S. aureus owing to their low sensitivity and specificity which are below the acceptable limits. Hence, PCR for mecA gene is the best method for detecting methicillin resistance in S. aureus with respect to accuracy, sensitivity, specificity, speed, and cost effectiveness. ~ Detection of Methicillin Resistance in Staphylococcus Aureus by Polymerase Chain Reaction and Conventional Methods: A Comparative Study.
http://www.jlponline.org/article.asp?issn=097...ast=Pillai
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Quote:
Nucleic acid amplification tests, such as the polymerase chain reaction (PCR), can be used to detect the mecA gene, (IT'S)is the most common gene that mediates oxacillin resistance in staphylococci.
However, mecA PCR tests will not detect novel resistance mechanisms such as mecC or uncommon phenotypes such as borderline-resistant oxacillin resistance. ~ CDC
http://www.cdc.gov/MRSA/lab/index.html
Quote:
The time taken for diagnosing MRSA by conventional methods is 48-72 h, which is more as compared to PCR which takes 18-24 h.
Conclusion: This study recommends advocating PCR for mecA gene on a regular basis for detecting methicillin resistance in S. aureus isolates isolated from sterile body fluids or from special units such as intensive care units.
......
.......
Laboratory diagnosis and susceptibility testing are crucial steps in treating, controlling, and preventing MRSA infections. Discrepancies in detection have an adverse effect on patient management, thereby highlighting the importance of accuracy in detection. Hence, methods used to detect MRSA in clinical samples should have high sensitivity and specificity and, most importantly, the result should be available within a short time. Various methods have evolved for rapid detection of methicillin-resistant staphylococci, but the optimal method for the detection remains controversial. The most commonly used method in the laboratories is culture and antibiotic sensitivity test (AST) [oxacillin disk diffusion (ODD)]. Other methods available for diagnosing MRSA include mannitol salt agar (MSA) with oxacillin (agar screening method), minimum inhibitory concentration (MIC) tests, agar dilution tests etc., All these tests are the conventional phenotypic methods of MRSA identification. [12],[13] Genotypic (molecular) method is the polymerase chain reaction (PCR) based method for detecting mecA gene, which remains the "gold standard" for diagnosing MRSA. [8],[13],[14]
.......
.......
Our study concludes that the commonly used phenotypic tests are not completely reliable for the detection of methicillin resistance in S. aureus owing to their low sensitivity and specificity which are below the acceptable limits. Hence, PCR for mecA gene is the best method for detecting methicillin resistance in S. aureus with respect to accuracy, sensitivity, specificity, speed, and cost effectiveness. ~ Detection of Methicillin Resistance in Staphylococcus Aureus by Polymerase Chain Reaction and Conventional Methods: A Comparative Study.
http://www.jlponline.org/article.asp?issn=097...ast=Pillai
buy shares is right!!!!
its MMTC!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
AND THIS IS THE THUNDERDOME!!!!!!!!!!!!!
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