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Posted On: 02/08/2024 11:39:08 AM
Post# of 22443
Is or does 1.0 picogram/ML detection really matter? A picogram is 1 trillionth of a gram! AI tells me......The detection limit for most compounds can be remarkably low, even reaching less than 1.0 picogram on the column. This sensitivity allows for precise analysis, especially in fields like mass spectrometry where minute quantities matter.
For instance, in Western blot analysis, researchers employ enhanced chemiluminescent (ECL) substrates to detect proteins. One such substrate is the Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate, which enables the detection of protein amounts ranging from picograms to high femtograms2.
Similarly, the Thermo Scientific Pierce ECL Western Blotting Substrate is another powerful tool. It allows the detection of picogram amounts of antigen using a luminol-based chemiluminescent reaction. This prolonged chemiluminescence can be visualized on X-ray film or an imaging system3. Researchers achieve optimal results by carefully adjusting primary and secondary antibody dilutions3.
In summary, the ability to detect such tiny quantities opens up exciting possibilities for scientific research and diagnostic.
For instance, in Western blot analysis, researchers employ enhanced chemiluminescent (ECL) substrates to detect proteins. One such substrate is the Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate, which enables the detection of protein amounts ranging from picograms to high femtograms2.
Similarly, the Thermo Scientific Pierce ECL Western Blotting Substrate is another powerful tool. It allows the detection of picogram amounts of antigen using a luminol-based chemiluminescent reaction. This prolonged chemiluminescence can be visualized on X-ray film or an imaging system3. Researchers achieve optimal results by carefully adjusting primary and secondary antibody dilutions3.
In summary, the ability to detect such tiny quantities opens up exciting possibilities for scientific research and diagnostic.
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