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Posted On: 06/13/2019 7:22:02 AM
Post# of 30066
High Capacity cDNA RT-kit and TaqManVR qRT-PCR analysis was done with Roche LightCycler 480 system. Data was analyzed by the 2-DCT method and the unpaired Student t-test …
SY Cheng, WF Chen, ZH Wen
Results: The expression levels for the 3 NTFs: mesencephalic astrocyte-derived neurotrophic factor (MANF), cerebral dopamine neurotrophic factor (CDNF) and growth differentiation factor (GDF15) were profiled from the TCGA and GTEx databases. MANF RNA was upregulated in 9 different cancers, CDNF was up-in 1 and downregulated in 5 cancers and GDF15 was up-in 14 and downregulated in 3 cancers. qRT-PCR for MANF and CDNF from 52 patients with CRC showed that MANF is significantly upregulated 2-fold (p< 0.001) in CRC as compared to controls from the same patients, and upregulation was already seen in tumor-nearby samples. The analysis of RNA samples from 38 BC patients showed significant down-regulation for CDNF (p< 0.05). Conclusions: These results support the hypothesis that NTFs indeed play a role in tumors and provide the basis for the need of further study for these factors within the cancer paradigm.
https://scholar.google.com/scholar?hl=en&...YglenAEMcJ
SY Cheng, WF Chen, ZH Wen
Results: The expression levels for the 3 NTFs: mesencephalic astrocyte-derived neurotrophic factor (MANF), cerebral dopamine neurotrophic factor (CDNF) and growth differentiation factor (GDF15) were profiled from the TCGA and GTEx databases. MANF RNA was upregulated in 9 different cancers, CDNF was up-in 1 and downregulated in 5 cancers and GDF15 was up-in 14 and downregulated in 3 cancers. qRT-PCR for MANF and CDNF from 52 patients with CRC showed that MANF is significantly upregulated 2-fold (p< 0.001) in CRC as compared to controls from the same patients, and upregulation was already seen in tumor-nearby samples. The analysis of RNA samples from 38 BC patients showed significant down-regulation for CDNF (p< 0.05). Conclusions: These results support the hypothesis that NTFs indeed play a role in tumors and provide the basis for the need of further study for these factors within the cancer paradigm.
https://scholar.google.com/scholar?hl=en&...YglenAEMcJ
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